rabbit polyclonal anti fabp4  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Vitamin D alleviates HFD-induced hepatic fibrosis by inhibiting DNMT1 to affect the TGFβ1/Smad3 pathway

doi: 10.1016/j.isci.2024.111262

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Article Snippet: Rabbit polyclonal anti-FABP4 , Cell Signaling Technology , Cat#50699.

Techniques: Recombinant, Methylation, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Sequencing, Negative Control, Positive Control, Software

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 1. Expression levels of FABP4 in sheep endometrium. (a) FABP4 mRNA expression in sheep endometrium on day 4 and day 15. (b, c) FABP4 protein expression in sheep endometrium on day 4 and 15. The optical density was normalized to the density of β-actin in the same lane. (d, e) Rabbit IgG group was used as the negative control for analyzing the localization and expression of FABP4 in sheep endometrium. Data are presented as mean ± standard error with significant differences at P < 0.05 and extremely significant differences at P < 0.01. * indicates P < 0.05, ** indicates P < 0.01, and no sign indicates that the difference is not significant. The technique was repeated thrice.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Negative Control

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 2. Expression of FABP4 in SEECs. (a) Isolation and purification of SEECs (250 ×). When the cells reached the sixth passage, they became larger and rounder and began to senesce. (b) Immunofluorescence identification of SEECs (CK18) (100 ×). (c) Localization of FABP4 in SEECs (Yellow arrows are FABP4 in the nuclei) (25 ×).

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Isolation, Purification, Immunofluorescence

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 3. Hormone treatment of SEECs and detection of changes in FABP4 expression. (a) Protein expression levels of PGR and ER after combined hormone treatment. (b) The mRNA expression levels of ISG15, HOXA10, CXCL10, and RSAD2 after hormone treatment. (c) Levels of the prostaglandin secreted from the SEECs after hormone treatment.(d) Expression levels of FABP4 after hormone treatment. All data are presented as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 4. FABP4 inhibition impedes SEEC function. (a, b) Mobility of SEECs at 0, 24, 48, 72 and 96 h was measured using a scratch test. Migratory capacity was calculated as a percentage of healing area relative to time 0. (c) CCK-8 viable cell counts quantified the proliferative capacity of SEECs treated with hormone and FABP4 inhibitor BMS309403. (d–g) Expression levels of EMT after hormone and inhibitor treatment (E-cadherin, N-cadherin, Vim, and β-catenin). (h, i) Changes in endoplasmic reticulum stress-related protein CHOP and GRP78 were measured. (j, k) Changes in autophagy- related proteins p-mTOR, LC3B II/I, and P62. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Inhibition, CCK-8 Assay, Expressing

Journal: The Journal of reproduction and development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Fig. 5. TG and 3-MA treatment partially restores SEEC function after BMS30940 suppression of FABP4. (a, b) Expression levels of key proteins in the endoplasmic reticulum stress signaling pathway after combined treatment with hormones, BMS309403, and TG. (c, d) Expression levels of key proteins in autophagy and apoptosis signaling pathways after combined treatment with hormones, BMS309403, and 3-MA. (e) Secreted prostaglandin levels from SEECs after combined treatment with hormones, BMS309403, and TG or 3-MA. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Protein-Protein interactions

Journal: iScience

Article Title: TET2 is recruited by CREB to promote Cebpb , Cebpa , and Pparg transcription by facilitating hydroxymethylation during adipocyte differentiation

doi: 10.1016/j.isci.2023.108312

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Article Snippet: Rabbit polyclonal anti-FABP4 , Proteintech , Cat#12802-1-AP; RRID: AB_2102442.

Techniques: Virus, Plasmid Preparation, Recombinant, Transfection, Lysis, CCK-8 Assay, BIA-KA, Cholesterol Assay, CRISPR, Software, Magnetic Beads

Journal: Oncology Reports

Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming

doi: 10.3892/or.2023.8578

Figure Lengend Snippet: Tumor-neighboring adipocytes promote PC progression. (A) Representative FABP4 staining of adipocytes in the PC tissue. (B) The size of adipocytes adjacent to or distant from pancreatic cancer cells. (C) Schematic illustration of the adipocyte-PC coculture model using a Transwell system. (D) The morphological changes of Panc-1 cells were assessed following coculture with 3T3-L1 mature adipocytes. (E and F) The PC cell lines Panc-1 and MIA PaCa2 were cultured with or without mature adipocytes. After 5 days, cancer cells were used for (E) migration and (F) Matrigel invasion assays. Representative images (left) and quantification (right) of three independent experiments are shown. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. PC, pancreatic cancer.

Article Snippet: The paraffin-embedded tissues were stained with rabbit anti-FABP4 polyclonal antibody (pAb; cat. no. 12802-1-AP; ProteinTech Group, Inc.) overnight at 4°C.

Techniques: Staining, Cell Culture, Migration, Standard Deviation